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Wikipedista:Julie Vacková/editujte s odvahou

Macrophage Polarization

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Macrophage polarization is a process when macrophage expresses different functional programs in response to microenvironmental signals.[1] There are lots of functional states of macrophage polarization and they can be fully polarized and acquire specific phenotype like M1 (classically activated macrophages) or M2 (alternatively activated macrophages). [2] These specific phenotypes depend on the tissue and specific microenvironment where macrophages are. [3] On one hand macrophage polarization is very important for host defense against pathogen, but on the other hand it is essential for maintenance of homeostasis. [4] Prolonged M1 type of macrophages is harmful for the organism and that is why tissue repair and restoration is necessary. M2 macrophages are responsible for that although they are connected with chronic infectious diseases. [5]

M1 macrophages

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Classically activated macrophages was named by Mackaness in the 1960s [6]. They express transcription factors like interferon-regulatory factor (IRF5), nuclear factor of kappa light polypeptide gene enhancer (NF-kB), activator protein 1 (AP-1) and STAT1. [7]Typical are pro – inflammatory molecules (e.g. IFN-γ, IL-12, IL-23, TNF, IL-6, IL-1, specific chemokines and antigen presentation molecules).

M1 macrophages are irreplaceable during acute infectious diseases and provide host protection against intracellular bacteria or viruses by production of nitric oxide (NO) or reactive oxygen intermediates (ROI). [8] [9] IFN-γ produced by Th1 lymphocytes is the most important cytokine which is responsible for classical macrophage activation. Natural killer (NK) cells and macrophages themselves produce IFN-γ as well. This cytokine regulates gene expression programs of macrophages like cytokine receptors, cell activation markers or cell adhesion molecules. For classical macrophage activation is necessary also lipopolysaccharide (LPS) – typical for gram negative bacteria – which is mainly recognised by TLR4, or lipoteichoic acid (LTA) – typical for gram positive bacteria. Granulocyte – macrophage colony stimulation factor (GM-CSF) stimulates M1 too. [10]

M2 macrophages

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Alternatively activated macrophages cover a continuum of functional states which are divided into groups – M2a, M2b and M2c macrophages. [11]There are different stimuli for each type – interleukin-4 (IL-4), IL-13 (M2a), immune complex + Toll-like receptor (TLR) or IL-1 receptor ligands (M2b), IL-10 and glucocorticoids (M2c).

M2a macrophages are connected with Th2 immune response. Th2 cells, eosinophils, basophils and macrophages themselves produce IL-4. They are important for encapsulation of parasites but they are also responsible for the type II hypersensitivity. For M2b is common increased IL-10 production but IL-12 production is turned off. Antigen presentation is upregulated (MHC II, CD86). M2c macrophages are important for secretion of IL-10, TGF-β. They also contribute on production of extracellular matrix components and tissue remodeling. Glucocorticoids influence their adherence, dissemination, apoptosis and phagocytosis of macrophage. [12]

Tumour associated macrophages

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Tumour associated macrophages (TAM) are typical for their protumoural functions like promotion of cancer cell motility, metastasis formation and angiogenesis [13] and their formation is dependent on microenvironmetal factors which are present in developing tumour. [14] TAMs produce immunosuppressive cytokines like IL-10, TGFβ and PGE2 very small amount of NO or ROI and low levels of inflammatory cytokines (IL-12, IL-1β, TNFα, IL-6). [15] Ability of TAMs to present tumour-associated antigens is decreased as well as stimulation of the anti-tumour functions of T and NK cells. Also TAMs are not able to lyse tumour cells. [16] Targeting of TAM may be a novel therapeutic strategy against cancer.

  1. ↑ Mantovani, Alberto, et al. "Macrophage polarization: tumour-associated macrophages as a paradigm for polarized M2 mononuclear phagocytes." Trends in immunology 23.11 (2002): 549-555.
  2. ↑ Hamilton, T. A. (2014). "Myeloid Colony Stimulating Factors as Regulators of Macrophage Polarization." Frontiers in Immunology
  3. ↑ Lawrence, Toby, and Gioacchino Natoli. "Transcriptional regulation of macrophage polarization: enabling diversity with identity." Nature reviews immunology 11.11 (2011): 750-761.
  4. ↑ Hamilton, Thomas A., et al. "Myeloid colony-stimulating factors as regulators of macrophage polarization." Frontiers in immunology 5 (2014).
  5. ↑ Benoit, Marie, Benoît Desnues, and Jean-Louis Mege. "Macrophage polarization in bacterial infections." The Journal of Immunology 181.6 (2008): 3733-3739.
  6. ↑ Mackaness GB: Cellular resistance to infection. J Exp Med 1962,116:381-406.
  7. ↑ Krausgruber, Thomas, et al. "IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses." Nature immunology 12.3 (2011): 231-238.
  8. ↑ Sica, Antonio, et al. "Macrophage polarization in tumour progression."Seminars in cancer biology. Vol. 18. No. 5. Academic Press, 2008.
  9. ↑ Martinez, Fernando O., and Siamon Gordon. "The M1 and M2 paradigm of macrophage activation: time for reassessment." F1000prime reports 6 (2014).
  10. ↑ Martinez, Fernando O., and Siamon Gordon. "The M1 and M2 paradigm of macrophage activation: time for reassessment." F1000prime reports 6 (2014).
  11. ↑ Mantovani, Alberto, et al. "The chemokine system in diverse forms of macrophage activation and polarization." Trends in immunology 25.12 (2004): 677-686.
  12. ↑ Martinez, Fernando O., and Siamon Gordon. "The M1 and M2 paradigm of macrophage activation: time for reassessment." F1000prime reports 6 (2014).
  13. ↑ Lewis, Claire E., and Jeffrey W. Pollard. "Distinct role of macrophages in different tumor microenvironments." Cancer research 66.2 (2006): 605-612.
  14. ↑ Sica, Antonio, et al. "Macrophage polarization in tumour progression."Seminars in cancer biology. Vol. 18. No. 5. Academic Press, 2008.
  15. ↑ Sica, Antonio, et al. Autocrine production of IL-10 mediates defective IL-12 production and NF-kappa B activation in tumor-associated macrophages. J Immunol. 2000 Jan 15;164(2):762-7.
  16. ↑ Sica, Antonio, et al. "Macrophage polarization in tumour progression."Seminars in cancer biology. Vol. 18. No. 5. Academic Press, 2008.
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